General Cell Based Assays
Eurofins Discoverx PathHunter eXpress GPR4 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0369E2ACP1L)
The PathHunter GPR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR4 and EA-tagged β-Arrestin-2. Activation of GPR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCRL2 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0333E2ACP1M)
The PathHunter CCRL2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19) based activation of CCRL2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCRL2 and EA-tagged β-Arrestin-2. Activation of CCRL2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NMBR Total Internalization Assay, 10pl Kit. (93-0939E3CP5L)
The PathHunter NMBR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of NMBR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged NMBR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the NMBR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Epoetin Alfa Bioassay Kit, 2-Plate. (93-0965Y3-00017)
The PathHunter Epoetin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses erythropoetin or epoetin alfa-induced dimerization of EPOR receptors. This product uses EFC technology. The cell line included overexpresses EPOR target protein fused to PK alongside EPOR target protein fused to EA. Upon ligand-induced dimerization of EPOR, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress TrkC Functional Assay, 10-Plate Kit. (93-0464E3CP16L)
The PathHunter eXpress TrkC Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkC dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkC and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PPYR1 (NPY4R) CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0233E2CP0M)
The PathHunter PPYR1 (NPY4R) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. pancreatic polypeptide) based activation of PPYR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PPYR1 and EA-tagged β-Arrestin-2. Activation of PPYR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MRGPRF CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0433E2ACP2L)
The PathHunter MRGPRF eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of MRGPRF GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRF and EA-tagged β-Arrestin-2. Activation of MRGPRF stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ENCAPSULA NANAOSCIENCE LLC MACROPHAGE DEPLETION KIT 2ML
Mannosylated Macrophage Depletion Kit (m-Clodrosome® + m-Encapsome®) 2ml
Enzo Life Sciences SOD activity kit (5x96wells)
ELS offers a large collection of superoxide dismutase products for oxidative stress research. This is a versatile assay for the determination of SOD activity. Differentiate between the activity of three SOD isoenzymes: MnSOD, FeSOD, and Cu/ZnSOD. Obtain higher throughput sample analysis with a convenient colorimetric 96 well plate format and fast results in just 10 minutes. Alternative Name: Superoxide dismutase. Applications: Colorimetric detection, Activity assay. Contents: Microtiter plate, Standard, SOD buffer concentrate, Xanthine oxidase, Xanthine solution, Triton x-100, WST-1 reagent
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 2-Plate. (95-0118Y2-00057)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress GPR35 Activated GPCR Internalization Assay, 10pl Kit. (93-0968E3CP5L)
The PathHunter GPR35 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GPR35 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR35 Activated GPCR Internalization Assay, 2pl Kit. (93-0968E3CP5M)
The PathHunter GPR35 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GPR35 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 15 Reagent, 100. (93-0563R15A)
AssayComplete Cell Plating 15 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx AssayComplete Cell Plating 13 Reagent, 500. (93-0563R13B)
AssayComplete Cell Plating 13 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx AssayComplete Cell Plating 20 Reagent, 100. (93-0563R20A)
AssayComplete Cell Plating 20 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.